Phospho-protein Staining
Phospho-protein Staining	Cy5 Total Protein Labeling	Image Overlay
Phospho-Protein Staining	Cy3 Total Protein Labeling	Image Overlay
2-D DIGE analysis of protein differential expression after the drug treatment followed by phospho-protein detection. 1. phospho-protein staining; 2. control; 3. drug treated.
Time-dependent phosphorylation changes of aconitate hydrase
ES Day 2 Day 4 Day6 Day8 Day10 Day 2 / ES Day 4 / ES Day 6 / ES Day 8 / ES Day 10 / ES Day 2 / ES Day 4 / Day 2 Day 6 / Day 4 Day 8 / Day 6 Day 10 / ES ES D2 / ES D4 / D2 D6 / D4 D8 / D6 D10 / D8 Spot # 20: phospho-Aconitate hydrase - 1.02 - 1.05 + 3.28 - 1.81 - 1.11 Spot # 21: phospho-Aconitate hydrase - 1.41 + 1.41 + 3.54 - 1.96 -1.06 Spot # 26: phospho-Aconitate hydrase - 1.45 + 1.91 + 1.50 - 1.09 - 1.03 Spot # 28: non-phospho-Aconitate hydrase - 1.45 +1.91 + 1.28 - 1.07 - 1.03
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