Fluorescent 2-color 2-D Western blot A: Protein Image B: Double-color Western Blots

Fluorescent 2-color 2-D Western blot Protein lysate was prepared from the mouse liver. 60 ug of protein sample was used for the minimal labeling by Cy2 and followed by 2-D electrophoresis (service provided by Applied Biomics, Hayward, California). After the 2-D gel image scan, the gel is subject to protein transfer followed by Western blot. A. 2-D gel image; B. Fluorescent 2-color Western blot. Western Blot was performed using rabbit anti-human keratin 8 and mouse monoclonal antibody against human keratin 18 as primary antibodies followed by using CF 555-labeled goat anti-rabbit Ig (Biotium, CA) and CF647-labeled goat anti-mouse Ig (Biotium, CA) as a secondary antibodies, and the Western Blot images were scanned using Typhoon scanner.

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