Protein ID by MALDI-TOF/TOF for DIGE customers 1

Protein ID by MALDI-TOF/TOF for External customers
(10 or less samples) 1

Protein ID by MALDI-TOF/TOF for External customers
( more than 10 samples) 1

LC-MS/MS on low complexity samples
(1D Gel band, 2 or less samples) 2

LC-MS/MS on moderate complexity samples 2

Phospho-peptide Enrichment

2. Turnaround time is 5-7 business days for >=3 samples, and up to 10 business days for <=2 samples. Please see procedure of LC-MS/MS for detailed steps of this service.

Low complexity samples: 1D gel band - Each sample should contain only one gel piece with one protein band, no bigger than 1x1x2mm in size. Samples with more than one gel piece, and/or more than one protein band will be considered as moderate to high complex samples. We recommend you cut out only the area with your protein of interest (1x1x2mm size) and follow these guidelines to prepare samples
Moderate complexity samples: protein mixture such as IP or column purified sample, <50 proteins
High complexity samples: more than 50 protein mix

Price covers the following:
1) Nano-LC coupled with auto-spotting
2) MS/MS
3) Standard database search against NCBI database using MASCOT
4) Data report (see an example)

Price does NOT cover: 1) sample preparation; 2) data request in addition to the standard report.

3. Turnaround time is 5-7 business days. Please see the procedure of Phospho-site Identification for detailed steps of this service. Price covers the identification of phosphorylation sites for 1 protein per sample, following are the major steps:

1) Reduction and alkylation
2) Trypsin digestion
3) Peptide extraction
4) Desalting
5) Phospho-peptide enrichment (if you order item # 321)
6) MALDI-TOF
7) MALDI-TOF/TOF on peptides matching the mass of phosphophorylated peptides from virtual digest
8) Database search against NCBI database using MASCOT
9) Data report: 1) top 10 hits via MASCOT search; 2) list of precursors with predicated phosphorylation sites; 3) MSMS spectrums on precursors showing loss of phosphate

Price does NOT cover: 1) protein identification; 2) sample preparation; 3) data request in addition to the standard report.

4. Turnaround time is 5-7 business days. Please see the procedure of Acetylation-site Identification for detailed steps of this service. Price covers the identification of acetylation sites for 1 protein per sample, following are the major steps:

1) Reduction and alkylation
2) Trypsin digestion
3) Peptide extraction
4) Desalting
5) MALDI-TOF
6) MALDI-TOF/TOF on peptides matching the mass of phosphophorylated peptides from virtual digest
7) Database search against NCBI database using MASCOT
8) Data report: 1) Top 10 hits via MASCOT search; 2) list of precursors with predicated acetylation sites; 3) MSMS spectrums on precursors showing an immonium ion peak at 126 m/z

Price does NOT cover: 1) protein identification; 2) sample preparation; 3) data request in addition to the standard report.

If customer does not provide protein sequence or accession number, an additional $150 will be charged for protein identification.