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Home > Proteomics Services > Phosphorylation and Other Post-Translational Modifications (PTM) 2D Western Blot
Phosphorylation and Other Post-Translational Modifications (PTM) 2D Western Blot
7. Biotinylation
Example
Proteins were extracted from human lung cancer cells. 2D DIGE gel was run on using pH 3-10 linear IPG strip and then transferred to the PVDF membrane.
A: Membrane 2D image was scanned after transfer
B: Protein biotinalytion (2) was revealed using CF647-labeled strepavidin (Biotium, Hayward, California)
C: Image overlay using ImageQuant software
A. Protein – Membrane image
B. Biotin blotting
Biotinalytion: Protein Image
Biotinalytion: Biotin blotting image

C. Protein + Biotin blotting overlay
Biotinalytion: Protein + Biotin blotting Image

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