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Home > Proteomics Services > Phosphorylation and Other Post-Translational Modifications (PTM) 2D Western Blot
Phosphorylation and Other Post-Translational Modifications (PTM) 2D Western Blot
2. Ubiquitination
Example.
Proteins were extracted from ovarian epithelial cells or mouse liver (treated after long-term feeding with 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC), using Applied Biomics 2D DIGE protocol.

Green color: Transferred protein; Red color: Anti-Ubiquitin.
A. Protein – Gel image (mouse liver)
B. Protein – Gel image (ovarian cells)

Ubiquitination: Protein Gel Image
Ubiquitination: Protein Gel Image

C. Anti-Ubiquitin Western Blot (mouse liver)

D. Anti-Ubiquitin Western Blot (ovarian cells)
Ubiquitination: Western Blot Image
Ubiquitination: Western Blot Image

E. Protein + WB overlay (mouse liver)

F. Protein + WB Overlay (ovarian cells)
Ubiquitination: Protein + Western Blot Image
Ubiquitination: Protein + Western Blot Image

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