Identification For Protein Mixture
NanoLC-MS/MS is a powerful technique used for many applications
which has very high sensitivity and specificity.
Our service focuses on protein identification of protein
complex ranging from 1D gel band to whole cell lysate.
This service is advantageous for protein mixtures because
prior fractionation on the nanoLC column will result in
the identification of more interesting or less abundant
proteins in your sample.
1) Low complexity samples: 1D gel band - Each
sample should contain only one gel piece with one protein
band, no bigger than 1x1x2mm in size. Samples with more
than one gel piece, and/or more than one protein band will
be considered as moderate to high complex samples. We recommend
you cut out only the area with your protein of interest
(1x1x2mm size) and follow these guidelines
to prepare samples.
2) Moderate complexity samples: protein mixture such
as IP or column purified sample, <100 proteins
3) High complexity samples: more than 100 protein mix