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PROTEOMICS SERVICES

LIQUID-BASED PROTEOMICS

iTRAQ® (2-8 Plex)
NanoLC-MS/MS

 

 
Home > Proteomics Services > NanoLC-MS/MS: Protein Identification For Protein Mixture
NanoLC-MS/MS: Protein Identification For Protein Mixture
NanoLC-MS/MS is a powerful technique used for many applications which has very high sensitivity and specificity.
Our service focuses on protein identification of protein complex ranging from 1D gel band to whole cell lysate.
This service is advantageous for protein mixtures because prior fractionation on the nanoLC column will result in the identification of more interesting or less abundant proteins in your sample.

Sample types:

1) Low complexity samples: 1D gel band - Each sample should contain only one gel piece with one protein band, no bigger than 1x1x2mm in size. Samples with more than one gel piece, and/or more than one protein band will be considered as moderate to high complex samples. We recommend you cut out only the area with your protein of interest (1x1x2mm size) and follow these guidelines to prepare samples.
2) Moderate complexity samples: protein mixture such as IP or column purified sample, <100 proteins
3) High complexity samples: more than 100 protein mix


1D Gel bands or Liquid samples
1D Gel bands or Liquid samples
Reduction/alkylation &
Trypsin Digestion
Reduction/alkylation &
Nano-HPLC
Nano-HPLC
MS/MS
MS/MS
Database Search for Protein ID
Database Search for Protein ID


Call our tech support at 510-887-0889 or send email to support@appliedbiomics.com with any technical questions.

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