Proteomics services: 2D Gel & Protein ID by Mass Spectrometry

		Call 510.887.0889 Email: support@appliedbiomics.com
Serving Your Proteomics Needs

2D DIGE SERVICES

2D DIGE Protein Profiling

Phospho-Protein Profiling

Serum 2D DIGE

2D Western Blot

2D Western Blot: Phospho/PTM

1D Quantitative Western Blot

Different Staining of 2D Gels

MASS SPEC SERVICES

Protein Identification

NanoLC-MS/MS

Identify Phospho-site

De Novo Peptide Sequencing

iTRAQ®

OTHER SERVICES

Pathway Analysis

Others

Identification of Phosphorylation Site

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Identification of Phosphorylation-site involves enriching phosphorylated peptides followed by mass spectrometry. The enrichment is critical since phosphorylated proteins often represent 1-2% of the total protein population. This service works best with purified phosphorylated proteins, and samples can be sent to us in-gel or in solution. In addition, customer will provide the protein sequence or accession number. If customer does not provide protein sequence or accession number, an additional $150 will be charged for protein identification.

Gel Bands/Spots or Liquid samples

Reduction/Alkylation & Trypsin Digestion

Phosphorylated Peptide Enrichment

MS/MS

Database Search for Phosphorylated Site Modification

Call our tech support at 510-887-0889 or send email to support@appliedbiomics.com with any technical questions.