This service works best with purified phosphorylated proteins,
samples can be sent to us in-gel or in solution. In addition,
customer will provide the amino acid sequence or accession
number of the target protein.
Identification of Phosphorylation-site on a single protein
involves enriching phosphorylated peptides followed by mass
spectrometry. The enrichment is critical since phosphorylated
proteins often represent 1-2% of the total protein population.
The presence of a phosphorylated residue is confirmed by the
MSMS peak showing the loss of phosphate.
Gel Bands/Spots or Liquid samples
Reduction/Alkylation & Trypsin Digestion
Phosphorylated Peptide Enrichment
Database Search for Phosphorylated Site Modification