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PROTEOMICS SERVICES

LIQUID-BASED PROTEOMICS

iTRAQ® (2-8 Plex)

MASS SPECTROMETRY

Identify Phosphorylation-site
Identify Acetylation-site
De Novo Peptide Sequencing

 

 

 

 

 

Home > Proteomics Services > Identification of Phosphorylation Site
Identification of Phosphorylation Site

This service works best with purified phosphorylated proteins, samples can be sent to us in-gel or in solution. In addition, customer will provide the amino acid sequence or accession number of the target protein.

Identification of Phosphorylation-site on a single protein involves enriching phosphorylated peptides followed by mass spectrometry. The enrichment is critical since phosphorylated proteins often represent 1-2% of the total protein population. The presence of a phosphorylated residue is confirmed by the MSMS peak showing the loss of phosphate.



Gel Bands/Spots or Liquid samples


Gel Bands/Spots or Liquid samples



Reduction/Alkylation & Trypsin Digestion

Reduction/Alkylation & Trypsin Digestion



Phosphorylated Peptide Enrichment

Phosphorylated Peptide Enrichment



MS
Mass Spectrometry


MS/MS
MS/MS



Database Search for Phosphorylated Site Modification

Call our tech support at 510-887-0889 or send email to support@appliedbiomics.com with any technical questions.


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