Two samples from Mouse liver, Control and Treated, were processed using Applied Biomics protocols

First, samples were run on 2D DIGE (pH4-7) and the gel images were scanned using Typhoon 9400 (A, B and C).
Second, proteins from 2D gel were transferred to the membrane and membrane images were scanned (D and E).
Third, Membrane was stained with Coomassie Blue (G).