iTRAQ® is a liquid-based proteomics technique allowing quantitative comparison between 2 (2-plex) to 8 (8-plex) samples using mass spectrometry (nanoLCMS/MS). This approach can provide both protein identification and protein ratios in one experiment. iTRAQ experiments work for the following samples:

1) Low complexity samples (<=10 protein)
2) Moderate complexity samples such as IP or column purified protein mixture (<=100 proteins)
3) High complexity samples, such as tissue, whole cell lysate and others
4) Very low protein amount (1-10 ug) which makes it un-suitable for other platforms