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Email: support@appliedbiomics.com
Serving Your Proteomics Needs


   


 

2D DIGE Analytical Gels

2D DIGE Global Protein Expression Profiling
2D DIGE & Phospho-Protein Expression Profiling
2D DIGE & Glyco-Protein Expression Profiling

2D DIGE Preparative Gel and Protein ID By Mass Spectrometry

Standard 2D Gels with Different Staining

2D Western Blot

1D Western Blot

1D DIGE

Mass Spectrometry Services

Protein ID by MALDI-TOF/TOF for DIGE customers
Protein ID by MALDI-TOF/TOF for External customers
LC-MS/MS on low complexity samples (1D Gel band)
LC-MS/MS on moderate complexity samples
LC-MS/MS on high complexity samples
Phospho-site Identification
De Novo peptide sequencing

iTRAQ®

Serum Protein Immuno-Depletion

Other Services

Customized and Contract Research

 




 

 

 

New Customer
$895 for one 2D DIGE gel
(2 Cydye labeling)


2D DIGE Global Protein Expression Profiling Analytical Gel

Services Description
Academic/Governmental Labs
Industrial/Commercial Labs
Code
Price
Code
Price
2D DIGE Analytical Gel (2 CyDye labeling)
101A2
$1200
101N2
$1500
2D DIGE Analytical Gel (3 CyDye labeling)
101A3
$1295
101N3
$1595


Price includes:
1) experimental design
2) customized sample preparation and protein concentration determination (2 samples for 2 CyDye labeling;3 samples for 3 CyDye labeling)
3) fluorescence dye labeling using CyDye
4) 1st dimension IEF and 2nd dimension SDS-PAGE
5) gel image scan using Typhoon scanner
6) In-gel analysis: Image analysis (ImageQuant) & Quantitative analysis of up to 30 spots (DeCyder)
7) Cross-gel analysis: Image analysis (ImageQuant) & Quantitative analysis of up to 30 spots, 2 comparisons for every 3 gels (DeCyder)

Data will be presented as following
1) ImageQuant analysis: Gel images of individual samples and overlay of two sample images (see an example)
2) DeCyder Analysis: Quantitative analysis and comparison of protein spots between different samples (see an example)

Price does NOT cover:
1) Manual matching spots between different gels
2) Screen shots of 3D view of each spot



2D DIGE & Phospho-Protein Expression Profiling Analytical Gel
Services Description
Academic/Governmental Labs
Industrial/Commercial Labs
Code
Price
Code
Price
2D DIGE & Phospho-Protein Profiling
401A-PP1
$1500
401N-PP1
$1800
Total Protein versus Phospho-Protein on 2D Image
401A-PP2
$1500
401N-PP2
$1800
Phospho-Protein Enrichment (from 5mg)
405A
$700
405N
$1000

2D DIGE & Glyco-Protein Expression Profiling Analytical Gel
Services Description
Academic/Governmental Labs
Industrial/Commercial Labs
Code
Price
Code
Price
2D DIGE & Glyco-Protein Profiling
420A-GP1
$1500
420N-GP1
$1800
Total Protein versus Glyco-Protein on 2D Image
420A-GP2
$1500
420N-GP2
$1800

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2D DIGE Preparative Gel and Protein ID By Mass Spectrometry
Services Description
Academic/Governmental Labs
Industrial/Commercial Labs
Code
Price
Code
Price
2D DIGE Preparative Gel (2 CyDye labeling)
102A2
$1200
102N2
$1500
2D DIGE Preparative Gel (3 CyDye labeling)
102A3
$1295
102N3
$1595

Spot Picking: up to 96 spots per gel

103
$200
103
$200
Protein ID by Mass Spectrometry (MALDI-TOF/TOF) 1
104A
$115 per spot
104N
$135 per spot
Pathways Analysis After Protein ID 2
203A
call for a quote
203N
call for a quote

1
. Prices discounted and only apply for customers doing 2D DIGE service with Applied Biomics. Turnaround time starting from Preparative gel to sending protein ID report is 7-10 business days. We offer high sensitivity protein identification from gel spots or bands using the latest technologies in mass spectrometry. In each run, four sensitivity standards in the amount of 1-10 femtomole are included. Our sensitivity is the range of 1-2 femtomole. Please see procedure of protein ID for detailed steps of this service. Price covers the following:
1) Gel treatment
2) In-gel trypsin digestion
3) Peptide extraction
4) Desalting
5) Spotting
6) MALDI-TOF
7) MALDI-TOF/TOF
8) Database search against NCBI database using MASCOT
9) Data report (see an example)

2. Pathway Analysis Service is provided only for customers doing the 2D DIGE and Protein ID services with Applied Biomics. The report will include the major pathway list with the following information:
1) Functional groups
2) Genes in each functional group
3) Enrichment score
4) P value and FDR


Standard 2D Gels with Different Staining
Services Description
Academic/Governmental Labs
Industrial/Commercial Labs
Code
Price
Code
Price

2D gel with 1 CyDye Labeling

401A
$800
401N
$960

2D gel with Coomassie Blue staining and
image scan

402A
$600
402N
$800
2D gel with Sypro Ruby staining and image scan
403A
$700
403N
$900

2D gel with Deep Purple staining and image scan

404A
$700
404N
$900
2D gel with Lumitein staining and image scan
405A
$600
405N
$800

*Sypro Ruby is a trademark of Invitrogen.



2D Western Blot
Services Description
Academic/Governmental Labs
Industrial/Commercial Labs
Code
Price
Code
Price
2D gel with 1 CyDye Labeling
401A
$800
401N
$960
2D DIGE gel with 2 CyDye labeling
101A2
$1200
101N2
1500

Transfer to PVDF or Nitrocellulose membrane 1

520A
$240
520N
$280
Western Blot with one color 2
512A
$400
512N
$480
Western Blot with two color 2
513A
$600
513N
$720

The following PTM primary antibodies are covered by Applied Biomics for the PTM-Western blot:

Anti-pTyr
Anti-pThr
Anti-pSer
Anti-Ubiquitin
Anti-Acetylated-Lysine
Anti-Sulfo-Tyrosine
Anti-Methyl-Arginine
Anti-Nitrotyrosine
Anti-Biotin

Customers are responsible for providing the other primary antibodies not listed above.
All the fluorescent labeled secondary antibodies are provided by Applied Biomics.

1. Price includes:
1)Trans-blotting 2D Gel to PVDF or Nitrocellulose membrane
2) Membrane images scanned after the transfer (see an example)

2. Price includes
3) Western blot using the primary antibodies provided by customers, Applied Biomics will provide secondary antibodies
4) Membrane images scanned after Western Blot (see an example)

Price does NOT cover the Primary antibodies


1D Western Blot (no Cydye labeling)
Services Description
Academic/Governmental Labs
Industrial/Commercial Labs
Code
Price
Code
Price
1D gel with no CyDye labeling / up to 14 samples 1
417A
$450
417N
$540
1D gel with 1 CyDye labeling / up to 14 samples 1
418A
$500
418N
$600

Transfer to PVDF or Nitrocellulose membrane 2

520A
$240
520N
$280
Western Blot with one color 3
512A
$400
512N
$480
Western Blot with two color 3
513A
$600
513N
$720
Customers are responsible for the primary antibodies, please call Tech Support to discuss details


1.
Price does NOT cover sample preparation

2.
Price includes:
1)Trans-blotting 2D Gel to PVDF or Nitrocellulose membrane
2) Membrane images scanned after the transfer

3. Price includes:
1) Western blot using the primary antibodies provided by customers, Applied Biomics will provide secondary antibodies
2) Membrane images scanned after Western Blot

Price does NOT cover the Primary antibodies


1D DIGE
Services Description
Academic/Governmental Labs
Industrial/Commercial Labs
Code
Price
Code
Price
1D DIGE gel with 2 CyDye labeling *
111A2
$600
111N2
$720
1D DIGE gel with 3 CyDye labeling *
112A3
$700
112N3
$840

* Price includes:
1) 1D Gel, up to 14 samples plus one lane for protein marker
2) 1D image scan and data report

Price does NOT cover sample preparation.


Mass Spectrometry Services
Services Description
Academic/Governmental Labs
Industrial/Commercial Labs
Code
Price/each sample
Code
Price/each sample

Protein ID by MALDI-TOF/TOF for DIGE customers 1

104A
$115/sample
104N
$135/sample

Protein ID by MALDI-TOF/TOF for External customers
(10 or less samples)
1

305A
$150/sample
305N
$180/sample

Protein ID by MALDI-TOF/TOF for External customers
( more than 10 samples) 1

305A
$135/sample
305N
$160/sample

LC-MS/MS on low complexity samples
(1D Gel band, 2 or less samples) 2

331A
$450/sample
331N
$540/sample
LC-MS/MS on low complexity samples
(1D Gel band, more than 2 samples) 2
331A
$350/sample
331N
$420/sample

LC-MS/MS on moderate complexity samples 2

332A
call for price
332N
call for price
LC-MS/MS on high complexity samples 2
333A
call for price
333N
call for price

Phospho-peptide Enrichment

321A
$150
321N
$180
Identification of Phosphorylation-site 3
322A
$375
322N
$450
De Novo Peptide Sequencing
340A
call for price
340N
call for price

1. Turnaround time is 2-5 business days. Please see procedure of protein ID for detailed steps of this service. Price covers the following:
1) Gel treatment
2) In-gel trypsin digestion
3) Peptide extraction
4) Desalting
5) Spotting
6) MALDI-TOF
7) MALDI-TOF/TOF
8) Standard database search against NCBI database using MASCOT
9) Data report (see an example)

2. Turnaround time is 5-7 business days for >=3 samples, and up to 10 business days for <=2 samples. Please see procedure of LC-MS/MS for detailed steps of this service.

Low complexity samples: 1D gel band - Each sample should contain only one gel piece with one protein band, no bigger than 1x1x2mm in size. Samples with more than one gel piece, and/or more than one protein band will be considered as moderate to high complex samples. We recommend you cut out only the area with your protein of interest (1x1x2mm size) and follow these guidelines to prepare samples
Moderate complexity samples: protein mixture such as IP or column purified sample, <50 proteins
High complexity samples: more than 50 protein mix

Price covers the following:
1) Nano-LC coupled with auto-spotting
2) MS/MS
3) Standard database search against NCBI database using MASCOT
4) Data report (see an example)

Price does NOT cover: 1) sample preparation; 2) data request in addition to the standard report.

3. Turnaround time is 5-7 business days. Please see the procedure of Identify Phospho-site for detailed steps of this service. Price covers the following:

1) Reduction and alkylation
2) Trypsin digestion
3) Peptide extraction
4) Desalting
5) Phospho-peptide enrichment (if you order item # 321)
6) MALDI-TOF
7) MALDI-TOF/TOF on peptides matching the mass of phosphophorylated peptides from virtual digest
8) Database search against NCBI database using MASCOT
9) Data report: 1) top 10 hits via MASCOT search; 2) list of precursors with predicated phosphorylation sites; 3) MSMS spectrums on precursors showing loss of phosphate

Price does NOT cover: 1) sample preparation; 2) data request in addition to the standard report.


If customer does not provide protein sequence or accession number, an additional $150 will be charged for protein identification.

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iTRAQ® Services
Services Description
Academic/Governmental Labs
Industrial/Commercial Labs
Code
Price/each sample
Code
Price/each sample

iTRAQ® Experiment

350A
call for price
350N
call for price


1. Turnaround time is 5-7 business days. Please see the procedure of iTRAQ® experiment for detailed steps of this service. Price covers the following:

1) Reduction and alkylation
2) Trypsin digestion
3) iTRAQ labeling followed by sample cleaning up
4) Nano-LC coupled with auto-spotting
5) MS and MS/MS
6) Standard database search against NCBI database using MASCOT
7) Data report

Price does NOT cover sample preparation.


Serum Protein Immuno-Depletion
Services Description
Academic/Governmental Labs
Industrial/Commercial Labs
Code
Price
Code
Price

Standard sample preparation for 2D DIGE from cell pellet, tissue, or protein precipitate.
Includes solubilization in lysis buffer and protein determination.

No charge
No charge
Major Protein Removal from Human Serum, Plasma, CSF, and Urine
601A
call for price
601N
call for price
IgY-12 immuno-depletion(Human 20 - 50 ul)
436A-12
call for price
436N-12
call for price
IgY-M7 immuno-depletion (Mouse 20 – 25 ul)
436A-M7
call for price
436N-M7
call for price
IgY-R7 immuno-depletion(Rat 20 – 35 ul)
436A-R7
call for price
436N-R7
call for price

IgY-12: against the 12 most abundant human plasma proteins: Human Serum Albumin, IgG, Fibrinogen, Transferrin, IgA, IgM, Haptoglobin, a2-Macroglobulin, a1-Acid Glycoprotein, a1-Antitrypsin and HDL (Apo A-I & Apo A-II).
IgY-M7: against the 7 most abundant rodent (mouse) plasma proteins: Mouse Serum Albumin, IgG, Fibrinogen, Transferrin, a1-antitrypsin, Haptoglobin & IgM.
IgY-R7: against the 7 most abundant rodent (rat or mouse) plasma proteins: Rat Serum Albumin, IgG, Fibrinogen, Transferrin, a1-antitrypsin, Haptoglobin & IgM.



Other Services
Services Description
Academic/Governmental Labs
Industrial/Commercial Labs
Code
Price
Code
Price
Additional data analysis (per hour)
202A
$100
202N
$120
Consulting Service (per hour)
201A
$100
201N
$200
Computerized 2D Gel Comparisons (2 gels)
801A
$450
801N
$550
Each additional gel
802A
$200
802N
$250
This analysis is for comparison of multiple scanned 2-D gel images. Requires the scanned gel images of previously run 2- D gels.
Biological Sample Storage  
First 6 months
No Charge
No Charge
Additional 6 month periods
701A
$125
701N
$150
Additional 12month periods
702A
$200
702N
$250
Electronic Data Storage(scans, images, etc.)  
First 120 days
No Charge
No Charge
On-file status for later retreival
703A
$125
703N
$150

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Customized and Contract Research


Whether you are an individual researcher, a university lab, or a commercial enterprise, Applied Biomics can help you to design a research program that will fit your laboratory's needs. For more information about our customization services and contract services, please contact us.

E-mail: support@appliedbiomics.com
Tel: 1-510-887-0889
Fax: 1-510-887-0867

 
 

Copyright ©2011 Applied Biomics, Inc.. All rights reserved. Terms