Proteomics: 2D DIGE & Mass Spectrometry

2D DIGE & Phospho-Protein Expression Profiling Analytical Gel

Services Description	Academic/Governmental Labs		Industrial/Commercial Labs
Services Description	Code	Price	Code	Price
2D DIGE & Phospho-Protein Profiling	401A-PP1	$1500	401N-PP1	$1800
Total Protein versus Phospho-Protein on 2D Image	401A-PP2	$1500	401N-PP2	$1800
Phospho-Protein Enrichment (from 5mg)	405A	$700	405N	$1000

2D DIGE & Glyco-Protein Expression Profiling Analytical Gel

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Services Description	Academic/Governmental Labs		Industrial/Commercial Labs
Services Description	Code	Price	Code	Price
2D DIGE & Glyco-Protein Profiling	420A-GP1	$1500	420N-GP1	$1800
Total Protein versus Glyco-Protein on 2D Image	420A-GP2	$1500	420N-GP2	$1800

2D DIGE Preparative Gel and Protein ID By Mass Spectrometry

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Services Description	Academic/Governmental Labs		Industrial/Commercial Labs
Services Description	Code	Price	Code	Price
2D DIGE Preparative Gel (2 CyDye labeling)	102A2	$1200	102N2	$1500
2D DIGE Preparative Gel (3 CyDye labeling)	102A3	$1295	102N3	$1595
Spot Picking: up to 96 spots per gel	103	$200	103	$200
Protein ID by Mass Spectrometry (MALDI-TOF/TOF) 1	104A	$115 per spot	104N	$135 per spot
Pathways Analysis After Protein ID 2	203A	call for a quote	203N	call for a quote
1. Prices discounted and only apply for customers doing 2D DIGE service with Applied Biomics. Turnaround time starting from Preparative gel to sending protein ID report is 7-10 business days. We offer high sensitivity protein identification from gel spots or bands using the latest technologies in mass spectrometry. In each run, four sensitivity standards in the amount of 1-10 femtomole are included. Our sensitivity is the range of 1-2 femtomole. Please see procedure of protein ID for detailed steps of this service. Price covers the following: 1) Gel treatment 2) In-gel trypsin digestion 3) Peptide extraction 4) Desalting 5) Spotting 6) MALDI-TOF 7) MALDI-TOF/TOF 8) Database search against NCBI database using MASCOT 9) Data report (see an example) 2. Pathway Analysis Service is provided only for customers doing the 2D DIGE and Protein ID services with Applied Biomics. The report will include the major pathway list with the following information: 1) Functional groups 2) Genes in each functional group 3) Enrichment score 4) P value and FDR

Standard 2D Gels with Different Staining

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Services Description	Academic/Governmental Labs		Industrial/Commercial Labs
Services Description	Code	Price	Code	Price
2D gel with 1 CyDye Labeling	401A	$800	401N	$960
2D gel with Coomassie Blue staining and image scan	402A	$600	402N	$800
2D gel with Sypro Ruby staining and image scan	403A	$700	403N	$900
2D gel with Deep Purple staining and image scan	404A	$700	404N	$900
2D gel with Lumitein staining and image scan	405A	$600	405N	$800

*Sypro Ruby is a trademark of Invitrogen.

2D Western Blot

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Services Description	Academic/Governmental Labs		Industrial/Commercial Labs
Services Description	Code	Price	Code	Price
2D gel with 1 CyDye Labeling	401A	$800	401N	$960
2D DIGE gel with 2 CyDye labeling	101A2	$1200	101N2	1500
Transfer to PVDF or Nitrocellulose membrane 1	520A	$240	520N	$280
Western Blot with one color 2	512A	$400	512N	$480
Western Blot with two color 2	513A	$600	513N	$720
The following PTM primary antibodies are covered by Applied Biomics for the PTM-Western blot: Anti-pTyr Anti-pThr Anti-pSer Anti-Ubiquitin Anti-Acetylated-Lysine Anti-Sulfo-Tyrosine Anti-Methyl-Arginine Anti-Nitrotyrosine Anti-Biotin Customers are responsible for providing the other primary antibodies not listed above. All the fluorescent labeled secondary antibodies are provided by Applied Biomics.
1. Price includes: 1)Trans-blotting 2D Gel to PVDF or Nitrocellulose membrane 2) Membrane images scanned after the transfer (see an example) 2. Price includes 3) Western blot using the primary antibodies provided by customers, Applied Biomics will provide secondary antibodies 4) Membrane images scanned after Western Blot (see an example) Price does NOT cover the Primary antibodies

1D Western Blot (no Cydye labeling)

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Services Description	Academic/Governmental Labs		Industrial/Commercial Labs
Services Description	Code	Price	Code	Price
1D gel with no CyDye labeling / up to 14 samples 1	417A	$450	417N	$540
1D gel with 1 CyDye labeling / up to 14 samples 1	418A	$500	418N	$600
Transfer to PVDF or Nitrocellulose membrane 2	520A	$240	520N	$280
Western Blot with one color 3	512A	$400	512N	$480
Western Blot with two color 3	513A	$600	513N	$720
Customers are responsible for the primary antibodies, please call Tech Support to discuss details
1. Price does NOT cover sample preparation 2. Price includes: 1)Trans-blotting 2D Gel to PVDF or Nitrocellulose membrane 2) Membrane images scanned after the transfer 3. Price includes: 1) Western blot using the primary antibodies provided by customers, Applied Biomics will provide secondary antibodies 2) Membrane images scanned after Western Blot Price does NOT cover the Primary antibodies

1D DIGE

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Services Description	Academic/Governmental Labs		Industrial/Commercial Labs
Services Description	Code	Price	Code	Price
1D DIGE gel with 2 CyDye labeling *	111A2	$600	111N2	$720
1D DIGE gel with 3 CyDye labeling *	112A3	$700	112N3	$840
* Price includes: 1) 1D Gel, up to 14 samples plus one lane for protein marker 2) 1D image scan and data report Price does NOT cover sample preparation.