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| 2D
DIGE Global Protein Expression Profiling Analytical Gel |
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Services Description
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Academic/Governmental
Labs
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Industrial/Commercial
Labs
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Code
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Price
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Code
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Price
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| 2D DIGE Analytical Gel (2 CyDye labeling) |
101A2
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$1200
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101N2
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$1500
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| 2D
DIGE Analytical Gel (3 CyDye labeling) |
101A3
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$1295
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101N3
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$1595
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Price includes:
1) experimental design
2) customized sample preparation and protein concentration
determination (2 samples for 2 CyDye labeling;3 samples for
3 CyDye labeling)
3) fluorescence dye labeling using CyDye
4) 1st dimension IEF and 2nd dimension SDS-PAGE
5) gel image scan using Typhoon scanner
6) image analysis using ImageQuant software and in-gel analysis
using DeCyder software (up to 3 hours/30 spots)
Data will be presented as following(see
an example)
1) ImageQuant analysis: Gel images of individual samples and
overlay of two sample images
2) DeCyder DIA Analysis: Quantitative analysis and comparison
of protein spots between different samples
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2D
DIGE & Phospho-Protein Expression Profiling Analytical
Gel
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Services Description
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Academic/Governmental
Labs
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Industrial/Commercial
Labs
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Code
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Price
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Code
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Price
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| 2D DIGE & Phospho-Protein Profiling |
401A-PP1
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$1500
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401N-PP1
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$1800
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| Total Protein
versus Phospho-Protein on 2D Image |
401A-PP2
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$1500
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401N-PP2
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$1800
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| Phospho-Protein Enrichment (from
5mg) |
405A
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$700
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405N
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$1000
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2D
DIGE & Glyco-Protein Expression Profiling Analytical Gel
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Services Description
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Academic/Governmental
Labs
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Industrial/Commercial
Labs
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Code
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Price
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Code
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Price
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| 2D DIGE & Glyco-Protein Profiling |
420A-GP1
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$1500
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420N-GP1
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$1800
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| Total Protein versus Glyco-Protein
on 2D Image |
420A-GP2
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$1500
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420N-GP2
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$1800
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Back To Top
| 2D
DIGE Preparative Gel and Protein ID By Mass Spectrometry |
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Services Description
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Academic/Governmental
Labs
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Industrial/Commercial
Labs
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Code
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Price
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Code
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Price
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| 2D DIGE Preparative Gel (2 CyDye
labeling) |
102A2
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$1200
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102N2
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$1500
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| 2D
DIGE Preparative Gel (3 CyDye labeling) |
102A3
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$1295
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102N3
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$1595
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Spot Picking: up to 96 spots per gel
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103
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$200
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103
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$200
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Protein ID by Mass Spectrometry (MALDI-TOF/TOF)
1
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104A
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$115 per spot
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104N
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$135 per spot
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| Pathways Analysis
After Protein ID 2 |
203A
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call for a quote
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203N
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call for a quote
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1. Prices discounted and only apply for customers doing
2D DIGE service with Applied Biomics. Turnaround time starting
from Preparative gel to sending protein ID report is 7-10
business days. We offer high sensitivity protein identification
from gel spots or bands using the latest technologies in mass
spectrometry. In each run, four sensitivity standards in the
amount of 1-10 femtomole are included. Our sensitivity is
the range of 1-2 femtomole. Please see procedure
of protein ID for detailed steps of this service. Price
covers the following:
1) Gel treatment
2) In-gel trypsin digestion
3) Peptide extraction
4) Desalting
5) Spotting
6) MALDI-TOF
7) MALDI-TOF/TOF
8) Database search against NCBI database using MASCOT
9) Data report (see
an example)
2. Pathway Analysis Service is
provided only for customers doing the 2D DIGE and Protein
ID services with Applied Biomics. The report will include
the major pathway list with the following information:
1) Functional groups
2) Genes in each functional group
3) Enrichment score
4) P value and FDR
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| Standard
2D Gels with Different Staining |
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Services Description
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Academic/Governmental
Labs
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Industrial/Commercial
Labs
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Code
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Price
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Code
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Price
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2D gel with 1 CyDye Labeling
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401A
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$700
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401N
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$900
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2D gel with Coomassie Blue staining
and
image scan
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402A
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$600
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402N
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$800
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| 2D gel with Sypro Ruby staining
and image scan |
403A
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$700
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403N
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$900
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2D gel with Deep Purple staining
and image scan
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404A
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$700
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404N
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$900
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| 2D gel with Lumitein staining and
image scan |
405A
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$600
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405N
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$800
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*Sypro Ruby is a trademark of Invitrogen.
| 2D
Gel Transfer to Membrane |
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Services Description
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Academic/Governmental
Labs
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Industrial/Commercial
Labs
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Code
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Price
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Code
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Price
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| 2D Gel Transfer to PVDF or Nitrocellulose
membrane |
520A
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$240
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520N
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$280
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Price includes:
1)Trans-blotting 2D Gel to PVDF or Nitrocellulose membrane
2) Membrane images scanned after the transfer (see
an example)
Price does NOT cover the following:
1) Running 2D or 2D DIGE gels
2) Shipping fee for sending the membrane back
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Services Description
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Academic/Governmental
Labs
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Industrial/Commercial
Labs
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Code
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Price
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Code
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Price
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| 2D Western Blot |
530A
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$640
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530N
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$760
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Customers are responsible for the primary
antibodies, please call Tech Support to discuss details
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Price includes:
1)Trans-blotting 2D Gel to PVDF or Nitrocellulose membrane
2) Membrane images scanned after the transfer (see
an example)
3) Western blot using the primary antibodies provided by customers
4) Membrane images scanned after Western Blot (see
an example)
Price does NOT cover the following:
1) Running 2D or 2D DIGE gels
2) Primary antibodies
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| Mass
Spectrometry Services |
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Services Description
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Academic/Governmental
Labs
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Industrial/Commercial
Labs
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Code
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Price
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Code
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Price
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Protein ID by MALDI-TOF/TOF for DIGE
customers 1
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104A
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$115
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104N
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$135
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Protein ID by MALDI-TOF/TOF for External
customers 1
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305A
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$150
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305N
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$180
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| LC-MS/MS on 1D Gel Band 2 |
331A
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$250
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331N
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$300
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LC-MS/MS on low to moderate complexity
samples 2
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332A
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$350
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332N
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$415
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| LC-MS/MS on high complexity samples
2 |
333A
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call for price
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333N
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call for price
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1. Turnaround time is 2-5 business
days. Please see procedure
of protein ID for detailed steps of this service. Price
covers the following:
1) Gel treatment
2) In-gel trypsin digestion
3) Peptide extraction
4) Desalting
5) Spotting
6) MALDI-TOF
7) MALDI-TOF/TOF
8) Database search against NCBI database using MASCOT
9) Data report (see
an example)
2. Turnaround time is 5 business
days. Please see procedure
of LC-MS/MS for detailed steps of this service. Price
covers the following:
1) Sample preparation including reduction and alkylation
2) Trypsin digestion
3) Nano-LC coupled with auto-spotting
4) MS/MS
5) Database search against NCBI database using MASCOT
6) Data report
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Back To Top
| Serum
Protein Immuno-Depletion |
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Services Description
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Academic/Governmental
Labs
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Industrial/Commercial
Labs
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Code
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Price
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Code
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Price
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Standard sample preparation for 2D DIGE from cell pellet,
tissue, or protein precipitate.
Includes solubilization in lysis buffer
and protein determination.
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No charge
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No charge
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Major Protein Removal
from Human Serum, Plasma, CSF, and Urine
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601A
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call for price
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601N
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call for price
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IgY-12 immuno-depletion(Human 20 - 50 ul)
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436A-12
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call for price
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436N-12
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call for price
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IgY-M7 immuno-depletion (Mouse 20 – 25
ul)
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436A-M7
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call for price
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436N-M7
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call for price
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IgY-R7 immuno-depletion(Rat 20 – 35 ul)
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436A-R7
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call for price
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436N-R7
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call for price
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IgY-12: against the 12 most abundant human plasma proteins:
Human Serum Albumin, IgG, Fibrinogen, Transferrin, IgA, IgM,
Haptoglobin, a2-Macroglobulin, a1-Acid Glycoprotein, a1-Antitrypsin
and HDL (Apo A-I & Apo A-II).
IgY-M7: against the 7 most abundant rodent (mouse) plasma
proteins: Mouse Serum Albumin, IgG, Fibrinogen, Transferrin,
a1-antitrypsin, Haptoglobin & IgM.
IgY-R7: against the 7 most abundant rodent (rat or mouse)
plasma proteins: Rat Serum Albumin, IgG, Fibrinogen, Transferrin,
a1-antitrypsin, Haptoglobin & IgM.
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Services Description
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Academic/Governmental
Labs
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Industrial/Commercial
Labs
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Code
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Price
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Code
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Price
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| Additional
data analysis (per hour) |
202A
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$100
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202N
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$120
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| Consulting
Service (per hour) |
201A
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$100
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201N
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$200
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| Computerized 2D Gel Comparisons
(2 gels) |
801A
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$450
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801N
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$550
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Each additional
gel
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802A
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$200
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802N
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$250
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This analysis is for comparison of multiple
scanned 2-D gel images. Requires the scanned gel images
of previously run 2- D gels.
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| Biological Sample Storage |
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First 6 months
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No Charge
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No Charge
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Additional 6 month periods
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701A
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$125
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701N
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$150
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Additional 12month periods
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702A
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$200
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702N
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$250
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| Electronic
Data Storage(scans, images, etc.) |
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First 120 days
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No Charge
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No Charge
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On-file status for later retreival
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703A
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$125
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703N
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$150
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Back To Top
Customized
and Contract Research
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Whether you are an individual researcher, a university lab,
or a commercial enterprise, Applied Biomics can help you to
design a research program that will fit your laboratory's
needs. For more information about our customization services
and contract services, please contact us.
E-mail: support@appliedbiomics.com
Tel: 1-510-887-0889
Fax: 1-510-887-0867
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2D
DIGE Analytical Gels
to
download the order form