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2D DIGE Analytical Gels

2D DIGE Global Protein Expression Profiling
2D DIGE & Phospho-Protein Expression Profiling
2D DIGE & Glyco-Protein Expression Profiling

2D DIGE Preparative Gel and Protein ID By Mass Spectrometry

Standard 2D Gels with Different Staining

2D Gel Transfer to Membrane

2D Western Blot

Mass Spectrometry Services

Protein ID by MALDI-TOF/TOF for DIGE customers
Protein ID by MALDI-TOF/TOF for External customers
LC-MS/MS on 1D Gel Band
LC-MS/MS on low to moderate complexity samples
LC-MS/MS on high complexity samples

Serum Protein Immuno-Depletion

Other Services

Customized and Contract Research

 




 

 

 


New Customer* Trial Offer

$850 for one 2D DIGE gel
(2 CyDye labeling)

Click here for more information

2D DIGE Global Protein Expression Profiling Analytical Gel

Services Description
Academic/Governmental Labs
Industrial/Commercial Labs
Code
Price
Code
Price
2D DIGE Analytical Gel (2 CyDye labeling)
101A2
$1200
101N2
$1500
2D DIGE Analytical Gel (3 CyDye labeling)
101A3
$1295
101N3
$1595

Price includes:
1) experimental design
2) customized sample preparation and protein concentration determination (2 samples for 2 CyDye labeling;3 samples for 3 CyDye labeling)
3) fluorescence dye labeling using CyDye
4) 1st dimension IEF and 2nd dimension SDS-PAGE
5) gel image scan using Typhoon scanner
6) image analysis using ImageQuant software and in-gel analysis using DeCyder software (up to 3 hours/30 spots)

Data will be presented as following(see an example)
1) ImageQuant analysis: Gel images of individual samples and overlay of two sample images
2) DeCyder DIA Analysis: Quantitative analysis and comparison of protein spots between different samples


2D DIGE & Phospho-Protein Expression Profiling Analytical Gel
Services Description
Academic/Governmental Labs
Industrial/Commercial Labs
Code
Price
Code
Price
2D DIGE & Phospho-Protein Profiling
401A-PP1
$1500
401N-PP1
$1800
Total Protein versus Phospho-Protein on 2D Image
401A-PP2
$1500
401N-PP2
$1800
Phospho-Protein Enrichment (from 5mg)
405A
$700
405N
$1000

2D DIGE & Glyco-Protein Expression Profiling Analytical Gel
Services Description
Academic/Governmental Labs
Industrial/Commercial Labs
Code
Price
Code
Price
2D DIGE & Glyco-Protein Profiling
420A-GP1
$1500
420N-GP1
$1800
Total Protein versus Glyco-Protein on 2D Image
420A-GP2
$1500
420N-GP2
$1800

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2D DIGE Preparative Gel and Protein ID By Mass Spectrometry
Services Description
Academic/Governmental Labs
Industrial/Commercial Labs
Code
Price
Code
Price
2D DIGE Preparative Gel (2 CyDye labeling)
102A2
$1200
102N2
$1500
2D DIGE Preparative Gel (3 CyDye labeling)
102A3
$1295
102N3
$1595

Spot Picking: up to 96 spots per gel

103
$200
103
$200
Protein ID by Mass Spectrometry (MALDI-TOF/TOF) 1
104A
$115 per spot
104N
$135 per spot
Pathways Analysis After Protein ID 2
203A
call for a quote
203N
call for a quote

1
. Prices discounted and only apply for customers doing 2D DIGE service with Applied Biomics. Turnaround time starting from Preparative gel to sending protein ID report is 7-10 business days. We offer high sensitivity protein identification from gel spots or bands using the latest technologies in mass spectrometry. In each run, four sensitivity standards in the amount of 1-10 femtomole are included. Our sensitivity is the range of 1-2 femtomole. Please see procedure of protein ID for detailed steps of this service. Price covers the following:
1) Gel treatment
2) In-gel trypsin digestion
3) Peptide extraction
4) Desalting
5) Spotting
6) MALDI-TOF
7) MALDI-TOF/TOF
8) Database search against NCBI database using MASCOT
9) Data report (see an example)

2. Pathway Analysis Service is provided only for customers doing the 2D DIGE and Protein ID services with Applied Biomics. The report will include the major pathway list with the following information:
1) Functional groups
2) Genes in each functional group
3) Enrichment score
4) P value and FDR


Standard 2D Gels with Different Staining
Services Description
Academic/Governmental Labs
Industrial/Commercial Labs
Code
Price
Code
Price

2D gel with 1 CyDye Labeling

401A
$700
401N
$900

2D gel with Coomassie Blue staining and
image scan

402A
$600
402N
$800
2D gel with Sypro Ruby staining and image scan
403A
$700
403N
$900

2D gel with Deep Purple staining and image scan

404A
$700
404N
$900
2D gel with Lumitein staining and image scan
405A
$600
405N
$800

*Sypro Ruby is a trademark of Invitrogen.


2D Gel Transfer to Membrane
Services Description
Academic/Governmental Labs
Industrial/Commercial Labs
Code
Price
Code
Price
2D Gel Transfer to PVDF or Nitrocellulose membrane
520A
$240
520N
$280
Price includes:
1)Trans-blotting 2D Gel to PVDF or Nitrocellulose membrane
2) Membrane images scanned after the transfer (see an example)

Price does NOT cover the following:
1) Running 2D or 2D DIGE gels
2) Shipping fee for sending the membrane back


2D Western Blot
Services Description
Academic/Governmental Labs
Industrial/Commercial Labs
Code
Price
Code
Price
2D Western Blot
530A
$640
530N
$760
Customers are responsible for the primary antibodies, please call Tech Support to discuss details
Price includes:
1)Trans-blotting 2D Gel to PVDF or Nitrocellulose membrane
2) Membrane images scanned after the transfer (see an example)
3) Western blot using the primary antibodies provided by customers
4) Membrane images scanned after Western Blot (see an example)

Price does NOT cover the following:
1) Running 2D or 2D DIGE gels
2) Primary antibodies


Mass Spectrometry Services
Services Description
Academic/Governmental Labs
Industrial/Commercial Labs
Code
Price
Code
Price

Protein ID by MALDI-TOF/TOF for DIGE customers 1

104A
$115
104N
$135

Protein ID by MALDI-TOF/TOF for External customers 1

305A
$150
305N
$180
LC-MS/MS on 1D Gel Band 2
331A
$250
331N
$300

LC-MS/MS on low to moderate complexity samples 2

332A
$350
332N
$415
LC-MS/MS on high complexity samples 2
333A
call for price
333N
call for price


1. Turnaround time is 2-5 business days. Please see procedure of protein ID for detailed steps of this service. Price covers the following:
1) Gel treatment
2) In-gel trypsin digestion
3) Peptide extraction
4) Desalting
5) Spotting
6) MALDI-TOF
7) MALDI-TOF/TOF
8) Database search against NCBI database using MASCOT
9) Data report (see an example)

2. Turnaround time is 5 business days. Please see procedure of LC-MS/MS for detailed steps of this service. Price covers the following:
1) Sample preparation including reduction and alkylation
2) Trypsin digestion
3) Nano-LC coupled with auto-spotting
4) MS/MS
5) Database search against NCBI database using MASCOT
6) Data report

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Serum Protein Immuno-Depletion
Services Description
Academic/Governmental Labs
Industrial/Commercial Labs
Code
Price
Code
Price

Standard sample preparation for 2D DIGE from cell pellet, tissue, or protein precipitate.
Includes solubilization in lysis buffer and protein determination.

No charge
No charge
Major Protein Removal from Human Serum, Plasma, CSF, and Urine
601A
call for price
601N
call for price
IgY-12 immuno-depletion(Human 20 - 50 ul)
436A-12
call for price
436N-12
call for price
IgY-M7 immuno-depletion (Mouse 20 – 25 ul)
436A-M7
call for price
436N-M7
call for price
IgY-R7 immuno-depletion(Rat 20 – 35 ul)
436A-R7
call for price
436N-R7
call for price

IgY-12: against the 12 most abundant human plasma proteins: Human Serum Albumin, IgG, Fibrinogen, Transferrin, IgA, IgM, Haptoglobin, a2-Macroglobulin, a1-Acid Glycoprotein, a1-Antitrypsin and HDL (Apo A-I & Apo A-II).
IgY-M7: against the 7 most abundant rodent (mouse) plasma proteins: Mouse Serum Albumin, IgG, Fibrinogen, Transferrin, a1-antitrypsin, Haptoglobin & IgM.
IgY-R7: against the 7 most abundant rodent (rat or mouse) plasma proteins: Rat Serum Albumin, IgG, Fibrinogen, Transferrin, a1-antitrypsin, Haptoglobin & IgM.



Other Services
Services Description
Academic/Governmental Labs
Industrial/Commercial Labs
Code
Price
Code
Price
Additional data analysis (per hour)
202A
$100
202N
$120
Consulting Service (per hour)
201A
$100
201N
$200
Computerized 2D Gel Comparisons (2 gels)
801A
$450
801N
$550
Each additional gel
802A
$200
802N
$250
This analysis is for comparison of multiple scanned 2-D gel images. Requires the scanned gel images of previously run 2- D gels.
Biological Sample Storage  
First 6 months
No Charge
No Charge
Additional 6 month periods
701A
$125
701N
$150
Additional 12month periods
702A
$200
702N
$250
Electronic Data Storage(scans, images, etc.)  
First 120 days
No Charge
No Charge
On-file status for later retreival
703A
$125
703N
$150

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Customized and Contract Research


Whether you are an individual researcher, a university lab, or a commercial enterprise, Applied Biomics can help you to design a research program that will fit your laboratory's needs. For more information about our customization services and contract services, please contact us.

E-mail: support@appliedbiomics.com
Tel: 1-510-887-0889
Fax: 1-510-887-0867

 
 

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