We offer the following services in serum 2-D DIGE:
A. Improved Serum 2-D DIGE without Immuno-depletion
B. Immuno-depletion of Serum Abundant Proteins
A.
Improved Serum 2-D DIGE Without Immuno-depletion
Applied Biomics has developed a protocol that significantly
improves the performance of standard protocol. For details
please click here to see our paper
published on Genetic Engineering & Technology News.
Please see below for examples using ABO (Applied Biomics)
improved serum 2-D DIGE protocol.
* Example 1: Human Serum Sample:
significantly more protein spots can be profiled by ABO
protocol.
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Standard 2-D protocol (pH3-10)
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ABO pH3-10 protocol
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ABO pH4-7 protocol
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Example
2: Analysis of Preclinical Trial Mouse Serum Before
and After Drug Feeding Using
ABO Protocol. Four paired mouse serum samples
were collected before and after 4 different treatments,
then each paired samples were analyzed by 2-D DIGE using
pH3-10 nonlinear IPG strip. The overlay images showed the
difference between the serum samples collected before (in
green color) and after (in
red color) the drug feeding
(18 hours). In the image overlay, the circled spots (in
red) indicated a protein with
increased abundance due to drug feeding. The 3-D view of
the same spot is generated by DeCyder software.
B. Immuno-depletion Of Serum Abundant Proteins For 2-D DIGE
The
Challenge of Biomarker Discovery from Plasma/Serum
Transferrin: 76 kDa
Fibrinogen: 65 kDa (a), 51 kDa (g)
a1-antitrypsin: 56 kDa
IgG: 150 kDa
HSA: 67 kDa
IgA: 180 kDa
IgM: 750 kDa
Transferrin: 79 kDa
a2-Macroglobulin: 160 kDa
Haptoglobin: 43 kDa
alpha 1-acid glycoprotein: 23 kDa
APO A-I, APO A-II: 23 kDa
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Protein dynamic range: 10 magnitude of difference in
amount |
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