Two samples from Mouse liver, Control and Treated, were
processed using Applied Biomics protocols
First,
samples were run on 2D DIGE (pH4-7) and the gel images were
scanned using Typhoon 9400 (A, B and C).
Second,
proteins from 2D gel were transferred to the membrane and
membrane images were scanned (D and E). Third,
Membrane was stained with Coomassie Blue (G).
Gel
Images
Membrane
Images
A. DIGE Gel image: Control sample
D. Membrane image (Control): scanned
after transfer
B. DIGE Gel image: Treated sample
E. Membrane image (Treated): scanned
after transfer
C. DIGE Gel Image Overlay
Green: Control Red:
Treated
F. Membrane Image Overlay: scanned after
transfer
Green: Control Red:
Treated
First,
samples were run on 2D DIGE (pH4-7) and the gel images were
scanned using Typhoon 9400 (A, B and C).
