Reproducibility 1: in-gel 2D DIGE and
across gel 2D DIGE
Reproducibility evaluation: same cell lysate sample was used
for different Cydye labeling.
This experiment was also repeated, and the image data was
analyzed by DeCyder software.
DeCyder
software analysis
Cy3/Cy2
Cy3/Cy5
Cy2/Cy5
Within Gel-1
2 S.D: after filter
Total spot number
Included spot number
Excluded spot number
1.22873
2235
915
1320
1.17503
2210
1070
1140
1.22577
2210
933
1277
Within Gel-2
2 S.D: after filter
Total spot number
Included spot number
Excluded spot number
1.21323
2256
1026
1230
1.18648
2250
1344
906
1.22116
2246
1.95
1151
Across Gels
2 S.D after filter
1.29214
1.25791
1.31146
Reproducibility 2: Stability of lysate stored at –
80 C.
Same cell lysate sample was thaw and freeze different times
followed by labeling by different minimal CyDye and 2D DIGE.
Highly
Reproducible
WT/S33A
(pair 1)
WT/S33A
(pair 2)
Two pairs of mouse liver protein expression
analyzed by 2D DIGE
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