Subcellular Proteomics

Subcellular proteomics with 2D DIGE focuses on different subcellular compartments, such as mitochondria, exosome, endoplasmic reticulum (ER), extracellular space, cytoplasm, nucleus and Golgi apparatus etc. We also offer nanoLC-MS/MS to study subcellular proteomics.

Applied Biomics developed unique methods to efficiently extract subcellular proteins from different subcellular fractions. In addition, we have optimized IEF and 2D electrophoresis conditions to achieve an optimal resolution of different subcellular proteins.

For gel layout, please view the study designs based on the total number of samples.

Example 1: Mitochondria proteome from mouse liver tissues.

The figure below shows 2D DIGE analysis of the Mitochondria proteins from 2 mouse liver tissue: Control and Treated. Mitochondria proteins were extracted from mitochondria fractions using Applied Biomics’ mitochondria protein extraction protocol. The bottom right image is a zoom-in view of an area with distinct red (up-regulated) and green (down-regulated) mitochondria protein spots.

Control

Mitochondria proteomics: Mitochondria proteome image (blue) from mouse liver - Control sample

Treated

Mitochondria proteomics: Mitochondria proteome image (blue) from mouse liver - Treated sample

Control / Treated

Mitochondria proteomics: Mitochondria proteome overlay image - Control / Treated

Control

Mitochondria proteomics: Mitochondria proteome image from mouse liver - Control sample

Treated

Mitochondria proteomics: Mitochondria proteome image from mouse liver - Treated sample

Control / Treated

Mitochondria proteomics: zoomed overlay image of Mitochondria proteome showing changed proteins between Control and Treated samples

Example 2: Exosomes proteome of human blood cell

pH 3-10 L

Exosome proteome image

Sample Info

Please follow these general principles regarding biohazardous materials.

Sample amount: 150-250 µg of subcellular fractions from each sample. This will be sufficient to cover the entire 2D DIGE procedure including analytical gel, preparative gel, spot picking and protein ID.

Protein concentration: 5-20 mg/ml is preferred.

Buffer: Please feel free to submit samples in whichever buffer that you would normally use. The standard sample preparation is covered by the DIGE gel cost. Such samples should have protein concentration in the range of 5-20 mg/ml. Additional charge may apply on samples that require extensive amount of the extra work such as protein eluting, concentrating and buffer exchange.

Pricing

Please view here for Subcellular Proteomics pricing.