Host Cell Protein Assay

It is challenging to detect and quantitate HCPs in the presence of products due to the high complexity of HCP mixtures. We offer HCP Assays by 2D DIGE and HCP Assays by NanoLCMSMS including HCP Antibody Coverage, HCP profiling and Product purity which allows high sensitivity detection and accurate quantitation of trace amounts of HCP contaminants in biopharmaceutical proteins.

Platform	2D DIGE-Based Host Cell Protein Assay						NanoLCMSMS-Based HCP Assay
Platform	1D DIGE	1D DIGE WB	2D Gel	2D WB	2D DIGE	2D DIGE WB	Nano-LCMSMS
Optional	Combined with Protein ID by MALDI-TOF/TOF or NanoLCMSMS						N/A

Separate HCP by	MW	MW	MW and pI	MW and pI	MW and pI	MW and pI	Hydrophobicity (& MW - optional)
Detection	CyDye label	CyDye label	Staining	Staining	CyDye label	CyDye label	MSMS
Sensitivity	0.2 ng	0.2 ng	1 ng	1 ng	0.2 ng	0.2 ng	10 amol
Visualize protein image							No
Protein and Antibody Signal on the same gel			No	No			No
Advantages	1) Quick comparison of HCPs in one dimension		1) Detailed comparison of HCPs in two dimensions 2) Accurate quantitation		1) Detailed comparison of HCPs in two dimensions 2) Accurate quantitation 3) High sensitivity 4) High consistency 5) High dynamic range		1) Detailed HCP components