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Host Cell Protein Assay

 

 

 

It is challenging to detect and quantitate HCPs in the presence of products due to the high complexity of HCP mixtures. We offer HCP Assays by 2D DIGE and HCP Assays by NanoLCMSMS including HCP Antibody Coverage, HCP profiling and Product purity which allows high sensitivity detection and accurate quantitation of trace amounts of HCP contaminants in biopharmaceutical proteins.

 

Platform
2D DIGE-Based Host Cell Protein Assay NanoLCMSMS-Based
HCP Assay
1D DIGE 1D DIGE WB 2D Gel 2D WB 2D DIGE 2D DIGE WB Nano-LCMSMS
Optional Combined with Protein ID by MALDI-TOF/TOF or NanoLCMSMS N/A


Separate HCP by

MW MW MW and pI MW and pI MW and pI MW and pI Hydrophobicity
(& MW - optional)


Detection

CyDye label CyDye label Staining Staining CyDye label CyDye label MSMS


Sensitivity

0.2 ng 0.2 ng 1 ng 1 ng 0.2 ng 0.2 ng 10 amol


Visualize protein image

No


Protein and Antibody Signal on the same gel

No No No


Advantages






1) Quick comparison of HCPs in one dimension





1) Detailed comparison of HCPs in two dimensions
2) Accurate quantitation




1) Detailed comparison of HCPs in two dimensions
2) Accurate quantitation
3) High sensitivity
4) High consistency
5) High dynamic range


1) Detailed HCP components