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Applied Biomics

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> Proteomics Services > Host Cell Protein (HCP) Assay: Spot Counting and Similarity

HCP Analysis: Spot Counting and Similarity
HCP Analysis: Antibody Coverage
HCP Analysis: Product Purity


Click to view a complete list of Applied Biomics HCP assays

Applied Biomics offers the most comprehensive Host Cell Protein detection analysis by using high resolution large format 2D DIGE platform. Our report provides individual HCP image and overlay image of different HCP samples, giving you the direct visualization and comparison of HCP compositions, modifications and fragmentations that no other platform could achieve. In addition, the quantitation analysis provides spot counting, similarity analysis, total HCP and DS quantity. Our LCMSMS and mass spectrometry services will provide the protein identity of HCPs. This platform has been successfully applied in the following areas:

1) Compare batch-to-batch HCP profile
2) Track purification process

Each analysis can test up to 3 samples simultaneously. For projects with more than 3 samples, Cross-gel analysis will be performed using customers pre-set control, or default internal standard. The report contains two parts.

A. Image analysis: Gel images of each sample and overlay of two sample images will be generated. In the 2-color overlay image, spots with green, red and yellow color indicate decreased, increased and similar expression, respectively.


Serum 2D DIGE

 Serum 2D DIGE

 Serum 2D DIGE


CHO Protein


Anti-CHO HCP Western Blot

B. Quantitative analysis:

- Spot counting of each sample: Total number of spots for each sample and presence/absence of each spot area
- Similarity analysis: Default or customer requested fold cutoff will be used to assess the % similarity between samples
- Drug Substance (DS) PPM calculation (per request)
- HCP PPM calculation (per request)