After completion of the 2D DIGE and protein ID experiments, we can perform pathway analysis on large protein sets of 30 or more proteins. Using the accession numbers provided in the Protein ID Report, we will cluster the proteins into functional groups using the Database for Annotation, Visualization, and Integrated Discovery (DAVID)

Identify differentially expressed spots using 2D DIGE
	Protein identification
	Import into DAVID for functional clustering