Acetylated-Lysine is a reversible PTM of proteins and plays a key role in regulating gene expression. Our Acetylated-Lysine 2D Western Blot platform offers high sensitivity detection and accurate quantitation of Lysine Acetylation in any protein samples. In addition, we offer mass spectrometry service to identify the Acetylated Lysine sites. Applied Biomics’ 2D Fluorescent Western Blot has been successfully applied in studying the functions of Acetylated-Lysine.

Representative PTM 2D Western Blots:

1. Phosphorylation	6. Nitrotyrosine
2. Ubiquitination	7. Biotinylation
3. Acetylated-Lysine	8. Succinyl-Lysine
4. Sulfo-Tyrosine	9. Citrullination
5. Methyl-Arginine	10. 4-hydroxynonenal (4-HNE)

3. Acetylated-Lysine

Example 1: Mouse liver

Proteins from drug treated Mouse liver were extracted, followed by Acetylated-Lysine 2D Western Blot using Applied Biomics’ protocols.

 

Mouse Liver Total Protein

Anti-Acetyllysine Western Blot

Total Protein / Anti-Acetyllysine

 

Example 2: Treated HEK293

 

Treated HEK293 Total Protein

>Anti-Acetyllysine Western Blot

Total Protein / >Anti-Acetyllysine

If you are interested, please follow these steps to obtain more information and order this service.