Biotinylated Protein 2D Western Blot: Detect & Quantify

Our Biotinylated Protein 2D Western Blot platform offers high sensitivity detection and accurate quantitation of Protein Biotinylation in any protein samples. Biotinylation is the process of covalently attaching biotin to a protein. Due to the strong affinity between biotin and streptavidin, the biotinylated proteins have been widely used to study protein-protein interactions.

Representative PTM 2D Western Blots:

1. Phosphorylation	6. Nitrotyrosine
2. Ubiquitination	7. Biotinylation
3. Acetylated-Lysine	8. Succinyl-Lysine
4. Sulfo-Tyrosine	9. Citrullination
5. Methyl-Arginine	10. 4-hydroxynonenal (4-HNE)

7. Biotinylation

Example: Human lung cancer cells

Proteins from Human lung cancer cells were extracted, followed by Biotinylated Protein 2D Western Blot using Applied Biomics’ protocols. Protein biotinalytion was revealed using CF647-labeled strepavidin (Biotium, Fremont, California).

Human Lung Cancer Cell Protein

Anti-Biotin Western Blot

Protein / Anti-Biotin Western Blot

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