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Succinyl Lysine 2D Western Blot: Detect & Quantify

 

 

 

Our Succinyl Lysine 2D Western Blot platform offers high sensitivity detection and accurate quantitation of Succinylated proteins in any protein samples. Lysine Succinylation (Ksucc) is a common post-translational modifications and occurs in a wide range of proteins. The addition of succinyl group changes lysine's charge from +1 to −1 and introduces a relatively large structural moiety (100 Da), bigger than acetylation (42 Da) or methylation (14 Da). Numerous proteins involved in the regulation of cellular and biological processes have been shown to be heavily succinylated. Emerging clinical data provides evidence that dysregulation of Ksucc is correlated with the development of cardiovascular diseases and cancer. Applied Biomics’ 2D Fluorescent Western Blot has been successfully applied in studying the functions of Succinylated protein.

Here are some representative applications:

1. Phosphorylation 6. Nitrotyrosine
2. Ubiquitination 7. Biotinylation
3. Acetylated-Lysine 8. Succinyl-Lysine
4. Sulfo-Tyrosine 9. Citrullination
5. Methyl-Arginine 10. 4-hydroxynonenal (4-HNE)



8. Succinyl-Lysine

Example: Mouse liver

Lysine succinylation (Ksucc) has a significant impact on protein folding and functions. Proteins from drug treated Mouse liver were extracted, followed by Succinyl Lysine 2D Western Blot using Applied Biomics’ protocols.

 

Mouse Liver Total Protein

PTM 2D Western Blot Lysine Succinylation

Anti-Succinyl-Lysine Western Blot

PTM 2D Western Blot Lysine Succinylation

Total Protein / Anti-Succinyl-Lysine Western

PTM 2D Western Blot Lysine Succinylation

If you are interested, please follow these steps to obtain more information and order this service.