Home > Proteomics Services > Ubiquitination Western Blot
Our Ubiquitinated Protein 2D Western Blot platform offers high sensitivity detection and accurate quantitation of Protein Ubiquitination in any protein samples. Ubiquitin is a small protein (8.6 kDa) found in most tissues of eukaryotic organisms. Ubiquitination is a process through which ubiquitin molecules are attached to protein substrates for protein degradation. Ubiquitination regulates the degradation, cellular location and activities of proteins. Applied Biomics’ 2D Fluorescent Western Blot has been successfully applied in studying the functions of Protein Ubiquitination.
Representative PTM 2D Western Blots:
1. Phosphorylation | 6. Nitrotyrosine |
2. Ubiquitination | 7. Biotinylation |
3. Acetylated-Lysine | 8. Succinyl-Lysine |
4. Sulfo-Tyrosine | 9. Citrullination |
5. Methyl-Arginine | 10. 4-hydroxynonenal (4-HNE) |
2. Ubiquitination
Example 1: Mouse liver
Proteins from drug treated Mouse liver were extracted, followed by 2D DIGE Western blot using Applied Biomics’ protocols.
Mouse liver protein |
Anti-Ubiquitin |
Protein / Anti-Ubiquitin |
Example 2: Ovarian cells
Ovarian cell protein |
Anti-Ubiquitin |
Protein / Anti-Ubiquitin |
If you are interested, please follow these steps to obtain more information and order this service.