HCP Contaminant Detection
Detecting and monitoring HCP Contaminant / product purity in the drug purification process is another important application of our HCP assay. Due to the different spot patterns from samples in the purification process, External Protein Markers (EPMs) can be added to the sample and used to adjust the HCP quantitation. Applied Biomics has developed several EPMs at different pH and Mw for you to choose from.
The EPMs can serve as a standard to accurately assess product purity in the following areas:
- Evaluating HCP Contaminant / product purity of therapeutic antibodies, recombinant proteins and vaccines
- Monitoring and optimizing of bioprocess
- Characterizing batch to batch cell lines
- Evaluating storage conditions of biological samples
Example 1: Adjust protein ratios by Applied Biomics EPMs
Example 2: Monitor HCP Contaminant / product purity by Applied Biomics EPMs
Applied Biomics External Protein Markers (EPMs)
Cy3-EPMs
Cy5-EPMs
Cy3-EPMs / Cy5-EPMs
- EPM_1: 76 kDa, pI=6.0, 6.2, 6.6
- EPM_2: 30 kDa, pI=5.4 – 6.0
- EPM_3: 14 kDa, pI=6.8 – 7.2
Locations of Applied Biomics EPMs Relative to HCPs
EPMs
Cell Lysates
EPMs / Cell Lysates
Example 1: Adjust protein ratios using Applied Biomics EPMs
No EPM Adjustment
With EPM Adjustment
| Spots | Ratios without EPM adjustment | Ratios with EPM adjustment |
| 1 | 1.6 | 3.9 |
| 2 | -6.3 | -2.6 |
| 3 | -18.6 | -7.6 |
| 4 | 9.2 | -22.5 |
| 5 | 6.4 | 15.7 |
| 6 | -11.8 | -4.8 |
| 7 | -2.2 | 1.1 |
| EPM | -2.4 | 1.0 |
Example 2: Monitor HCP Contaminant / product purity using Applied Biomics EPMs
DeCyder image of a process sample
Calculate HCP Contaminant / product purity
- Use DIGE Western blot information to locate all product protein (and their isoforms) – blue spots
- Calculate % HCP Contaminant /purity of Product in purification process
- Optional: Adjust the product ratios between different purification steps using EPMs – green spots
Sample Info
Please follow these general principles regarding biohazardous materials and buffer condition in getting your samples ready.
Protein amount for each gel: 20-50 µg of total protein from each sample.
Protein concentration: 5-20 mg/mL
Buffer: Please feel free to submit samples in whichever buffer that you would normally use. The sample preparation including protein extraction (if applicable), sample cleaning and measuring protein concentration is not covered by the 2D DIGE gel cost. Additional charge may apply on samples with concentration less than 4 mg/mL, or require significant amount of the extra work such as protein eluting, sample pooling, extensive buffer exchange or serum abundant protein depletion.
Sample type: Please inform our scientists about your sample type. We will send you additional tips for each sample type.
Pricing
| Services Description | Academic/Governmental Labs | Industrial/Commercial Labs | ||
|---|---|---|---|---|
| Code | Price | Code | Price | |
| 2D DIGE Analytical Gel (2 CyDye labeling) | 101A2 | $1259 | 101N2 | $1569 |
| 2D DIGE Analytical Gel (3 CyDye labeling) | 101A3 | $1399 | 101N3 | $1769 |
| HCP Quantitative Analysis | 202A-HCP | call for price | 202N-HCP | call for price |
Price covers:
- Experimental design
- Measure protein concentration
- Fluorescence dye labeling using CyDye (2 samples for 2 CyDye labeling; 3 samples for 3 CyDye labeling)
- 1st dimension IEF and 2nd dimension SDS-PAGE
- Gel image scan using Typhoon scanner
- Two hour data analysis covering image and DeCyder analysis
- Data report