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Reproducibility
Reproducibility 1: in-gel 2D DIGE and across gel 2D DIGE
Reproducibility evaluation: same cell lysate sample was used for different CyDye labeling.
This experiment was also repeated, and the image data was analyzed by DeCyder software.
DeCyder software analysis
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Cy3/Cy2
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Cy3/Cy5
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Cy2/Cy5
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| Within Gel-1 | |||
| 2 S.D: after filter Total spot number Included spot number Excluded spot number |
1.22873
2235 915 1320 |
1.17503
2210 1070 1140 |
1.22577
2210 933 1277 |
| Within Gel-2 |
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| 2 S.D: after filter Total spot number Included spot number Excluded spot number |
1.21323
2256 1026 1230 |
1.18648
2250 1344 906 |
1.22116
2246 1.95 1151 |
| Across Gels | |||
| 2 S.D after filter |
1.29214
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1.25791
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1.31146
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Reproducibility 2: Stability of lysate stored at – 80 C. Same cell lysate sample was thaw and freeze different times followed by labeling by different minimal CyDye and 2D DIGE.
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Highly Reproducible
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WT/S33A (pair 1)
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WT/S33A (pair 2)
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Two pairs of mouse liver protein expression analyzed by 2D DIGE