2D DIGE Data Analysis: In-Gel

The 2D DIGE In-Gel Data Analysis is ideal for comparing up to 3 samples, which can fit into one 2D DIGE gel. If your project involves comparing 4 or more samples, 2D DIGE Cross-Gel Analysis will be used. A typical 2D DIGE data analysis generates two main reports:

A. 2D DIGE Image Data Report by ImageQuant:

Gel images of individual samples and overlay of two sample images will be generated. In the overlay image, spots with green, red and yellow color indicate decreased, increased and similar expression, respectively.

WT

2D DIGE in-gel data analysis: gel image of WT sample from ImageQuant

Mutant

2D DIGE in-gel data analysis: gel image of Mutant sample from ImageQuant

WT / Mutant

2D DIGE in-gel data analysis: overlay image of WT and Mutant sample from ImageQuant

B. 2D DIGE Quantitation Data Report by DeCyder: 

Quantitative analysis and comparison of all spots between different samples. The differential protein expression is assessed by Differential In-gel Analysis (DIA), giving the high accuracy and reproducibility. Here is a screenshot of DeCyder window, which contains:

  1. General statistic data (2 S.D. value, total spot number detected, number of decreased and increased spots etc.)
  2. Protein expression ratio (fold of change)
  3. 3D image view of the corresponding spot
2D DIGE in-gel data analysis: Quantitation by DeCyder including spot map, spot distribution, 3D view and protein ratio table

Example: In-gel Image Analysis Using DeCyder Software

2D DIGE in-gel data analysis: DeCyder screeshot showing a spot up-regulated in Mutant comparing to WT