2D DIGE vs 2D Gel

Why run 2D DIGE instead of 2D gel electrophoresis?

In 2D gel, proteins are separated by PI (isoelectric point) in IEF (1^st dimension) and molecular weight in SDS PAGE (2^nd dimension).

2D DIGE is an improved version of standard 2D gel electrophoresis. In addition to all the features of 2D gel, 2D DIGE labels different samples with up to 3 fluorescent tags. This allows up to 3 samples to be run on the same gel—saving cost, minimizing gel-to-gel variation, and having higher sensitivity/accuracy.

Advantages of 2D DIGE over 2D Gel electrophoresis:

• Higher sensitivity: 2D DIGE uses Fluorescent labeling with a sensitivity of 0.2 ng/spot, versus the sensitivity of standard 2D gel electrophoresis with Coomassie blue staining at 100 ng/spot or silver staining at 1 ng/spot
• Higher accuracy: The extremely high spot resolution enables accurate spot quantitation. Differences in protein expression as small as 10% can be detected
• Higher reproducibility: Nearly identical data was obtained on the same sample labeled with different CyDye on the same gel or cross different gels, thus eliminating the need of running technical replicates
• Broader spectrum: Our large 2D DIGE gel format resolve ~5000 protein spots, allowing a wide dynamic range detection/quantitation of low abundant proteins, large proteins and small peptides
• Fewer number of gels: Each 2D DIGE gel can accommodate up to 3 different samples, versus 1 sample on each 2D Gel. For the same number of samples, only 1/3 number of 2D DIGE gels is needed.
• Lower cost: Our price is based on each gel instead of each sample. Lower number of 2D DIGE gels and no need of technical replicates translates into higher quality data at a lower cost than standard 2D Gel service. Price covers from sample preparation to publication-ready data.
• Fast turn-around: Results In 5-7 Days.