2D DIGE vs 2D Gel
Why run 2D DIGE instead of 2D gels?
In 2D-DIGE (two-dimensional difference in gel electrophoresis), we use fluorescent dyes to label proteins, allowing us to run up to 3 samples in one gel. There are many advantages to 2D-DIGE over traditional 2D gels:
| 2D DIGE | Standard 2D | |
| Staining Method | Fluorescent Dye | Staining |
| Spot Sensitivity | 0.2 ng/spot | Coomassie: 50 ng/spot Silver: 1 ng/spot Sypro Ruby: 1 ng/spot |
| Number of samples per gel | 3 per gel | 1 per gel |
| Number of in-gel comparisons | 3 comparisons per gel | 1 comparison per 2 gels |
| Spot resolution | Higher | Lower |
| Reproducibility | Higher | Lower |
- Higher sensitivity: Fluorescent dyes have a sensitivity of 0.2 ng/spot, versus the sensitivity of coomassie at 100 ng/spot or silver staining at 1 ng/spot
- Higher accuracy: The extremely high spot resolution enables accurate spot quantitation. Differences in protein expression as small as 10% can be detected
- Higher reproducibility: Nearly identical data was obtained on the same sample labeled with different Cydye on the same gel or cross different gels, thus eliminating the need of running technical replicates.
- Broader spectrum: Each gel can resolve ~5000 protein spots, allowing a wide dynamic range detection/quantitation of low abundant proteins, large proteins and small peptides.
- Fewer number of gels: Each gel can accommodate up to 3 different samples
- Lower cost: Our price is per gel (up to 3 samples can be loaded on 1 gel) not per sample. Price covers from sample preparation to publication-ready data, and complimentary consultation with your project leader
- Fast turn-around: 1 week
