2D DIGE vs 2D Gel
Why run 2D DIGE instead of 2D gels?
In 2D-DIGE (two-dimensional difference in gel electrophoresis), we use fluorescent dyes to label proteins, allowing us to run up to 3 samples in one gel. There are many advantages to 2D-DIGE over traditional 2D gels:
|2D DIGE||Standard 2D|
|Staining Method||Minimal Dye||Staining|
|Spot Sensitivity||0.2 ng/spot||50/ng/spot (Coomassie)|
1 ng/spot (Silver)
Spyro Ruby: 1 ng/spot
|Number of samples per gel||3 per gel||1 per gel|
|Number of in-gel comparisons||3 comparisons per gel||1 comparison per 2 gels|
- Higher sensitivity: Fluorescent dyes have a sensitivity of 0.2 ng/spot, versus the sensitivity of coomassie at 100 ng/spot or silver staining at 1 ng/spot
- Higher accuracy: The extremely high spot resolution enables accurate spot quantitation. Differences in protein expression as small as 10% can be detected
- Higher reproducibility: Nearly identical data was obtained on the same sample labeled with different Cydye on the same gel or cross different gels, thus eliminating the need of running technical replicates.
- Broader spectrum: Each gel can resolve ~5000 protein spots, allowing a wide dynamic range detection/quantitation of low abundant proteins, large proteins and small peptides.
- Fewer number of gels: Each gel can accommodate up to 3 different samples
- Lower cost: Our price is per gel (up to 3 samples can be loaded on 1 gel) not per sample. Price covers from sample preparation to publication-ready data, and complimentary consultation with your project leader
- Fast turn-around: 1 week