2D DIGE vs 2D Gel

Why run 2D DIGE instead of 2D gels?

In 2D-DIGE (two-dimensional difference in gel electrophoresis), we use fluorescent dyes to label proteins, allowing us to run up to 3 samples in one gel. There are many advantages to 2D-DIGE over traditional 2D gels:

2D DIGEStandard 2D
Staining MethodFluorescent DyeStaining
Spot Sensitivity0.2 ng/spot50/ng/spot (Coomassie)
1 ng/spot (Silver)
Spyro Ruby: 1 ng/spot
Number of samples per gel3 per gel1 per gel
Number of in-gel comparisons3 comparisons per gel1 comparison per 2 gels
Spot resolutionHigherLower
  • Higher sensitivity: Fluorescent dyes have a sensitivity of 0.2 ng/spot, versus the sensitivity of coomassie at 100 ng/spot or silver staining at 1 ng/spot
  • Higher accuracy: The extremely high spot resolution enables accurate spot quantitation. Differences in protein expression as small as 10% can be detected
  • Higher reproducibility: Nearly identical data was obtained on the same sample labeled with different Cydye on the same gel or cross different gels, thus eliminating the need of running technical replicates.
  • Broader spectrum: Each gel can resolve ~5000 protein spots, allowing a wide dynamic range detection/quantitation of low abundant proteins, large proteins and small peptides.
  • Fewer number of gels: Each gel can accommodate up to 3 different samples
  • Lower cost: Our price is per gel (up to 3 samples can be loaded on 1 gel) not per sample. Price covers from sample preparation to publication-ready data, and complimentary consultation with your project leader
  • Fast turn-around: 1 week