HCP Profiling Analysis by 1D SDS PAGE
Our HCP Profiling analysis by 1D gel can detect, quantify and compare HCP compositions of up to 10 HCP samples in 1 test. This platform has been successfully applied in the following areas:
- Quickly compare batch-to-batch HCP profile
- Track purification process
The HCP analysis by 1D gel offers a quick profiling and semi-quantitation of HCPs by Molecular weight. For comprehensive HCP profiling, please view our HCP Profiling Analysis by 2D.
The data report contains two parts:
- A. HCP Image Analysis: Direct visualization and comparison of HCP profile in 1 dimension.
- B. HCP Quantitative Analysis: Band detection; volume and percentage volume of each band
A. HCP Image analysis
1D gel images of HCPs separated by molecular weight. Since the samples are labeled with fluorescent CyDye, both black/white and color images will be provided.
Example: Image analysis of 2 HCP samples by 1D SDS-PAGE
Black / White HCP Image
Color HCP Image
B. HCP Quantitative analysis
The following quantification analysis can be performed to assess and compare the global protein profiling of HCP samples:
- Band detection
- Volume of each band
- Percentage volume of each band
Example: 1D Quantification analysis of HCP samples
1D HCP Quantitation Analysis Image
Please follow these general principles regarding biohazardous materials and buffer condition in getting your samples ready.
Protein amount for each gel: 20-50 µg of total protein from each sample.
Protein concentration: 5-20 mg/mL
Buffer: Please feel free to submit samples in whichever buffer that you would normally use. The standard sample preparation is covered by the DIGE gel cost. Such samples should have protein concentration in the range of 5-20 mg/ml. Additional charge may apply on samples that require extensive amount of the extra work such as protein eluting, concentrating, buffer exchange or serum abundant protein depletion.
Sample type: Please inform our scientists about your sample type. We will send you additional tips for each sample type.
|Services Description||Academic/Governmental Labs||Industrial/Commercial Labs|
|1D Gel (up to 10 samples) 1||418A||$549||418N||$649|
|HCP Quantitative Analysis||202A-HCP||TBD||202N-HCP||TBD|
1. Price does NOT cover sample preparation or CyDye labeling