1D and 2D HCP Profiling

We offer comprehensive HCP analysis to detect and quantify HCPs in the high complexity samples containing both HCPs and Drug Substance (DS). Our HCP Assay by 2D DIGE, 2D WesternBlot and HCP Assays by Nano LC-MS/MS platforms include HCP Antibody Coverage, HCP profiling and Product purity. These HCP analyses allow high sensitivity detection and accurate quantitation of trace amounts of HCP contaminants in biopharmaceutical proteins.

HCP Assays by 2D DIGE

Platform	Separate by	Detection	Sensitivity	Protein image	Protein/WB in 1 gel	Advantages
1D DIGE	MW	CyDye label	0.2 ng			1) Quick comparison of HCPs in one dimension
1D DIGE WB	MW	CyDye label	0.1 ng
2D Gel	MW and pI	Staining	1-100 ng			1) Detailed comparison of HCPs in two dimensions 2) Accurate quantitation
2D WB	MW and pI	Staining	0.1 ng
2D DIGE	MW and pI	CyDye label	0.2 ng			1) Detailed comparison of HCPs in two dimensions 2) Accurate quantitation 3) High sensitivity 4) High consistency 5) High dynamic range
2D DIGE WB	MW and pI	CyDye label	0.1 ng

HCP Assays by Nano LC-MS/MS

Platform	Separate	Detection	Sensitivity	Protein image	Protein/WB in 1 gel	Advantages
NanoLC MS/MS	Hydrophobicity & MW-optional	MS/MS	10 amol			1) Quick comparison of HCPs in one dimension