News & Featured Publications

Featured Publication: Kinase Substrate Mapping Using Phospho-Proteomics Platform

September 19, 2022

A recent publication in Microbiology Spectrum used our PhosphoProteomics platform to identify important kinase substrates forProtein Kinase K (PknK) in Mycobacterium tuberculosis.

Pknk plays a key role in regulating the growth of M. tuberculosis. The PknK knock out strain showed increased survival under metabolic stress such as carbon and nitrogen starvation signals, compared to WT strains.

To identify Pknk substrates, authors compared the global phosphoproteomic profiles of WT and phosphorylation-defective PknK strains. Both the phosphorylation and protein level of each protein was assessed. Among thousands of proteins, only 22 spots showed positive phosphorylation level in the WT but not the Pknk-defective strain, as well as protein level change.

Further mass spectrometry of the 18 spots identified Pknk substrates such as Rho transcription terminator and several critical enzymes involved in metabolism and signal transduction.

Together with other findings, this study further established the Pknk signal transduction network and its central role for mycobacterial survival.

Comments: It is challenging to study protein phosphorylation due to the low phosphor-level and lack of effective separation between phosphor-protein and native protein. Our PhosphoProteomics platform addresses both these issues through fast screening and quantification of phospho-proteins with high sensitivity and resolution. The authors in this publication were able to quickly narrow down their targets to 22 protein spots among thousands of proteins. More importantly, proteins identified using our platform were validated by transcriptome and in vivo analysis.

Featured Service: Choosing the right sample type for HCP Antibody Coverage

August 17, 2022

It is critical to detect and measure HCPs in therapeutic products. However, a common drawback in many assays is the inability to detect protein modifications and degradations.

Our HCP Antibody Coverage platform can detect HCP composition, protein modifications, and degradations with high sensitivity and consistency – making it advantageous to ELISA, AAE and standard 2D Western blot. These features can greatly improve the purification steps during manufacturing and purity analysis of the final drug substance (DS).

While high quality HCP antibody is critical for optimal HCP coverage, it is equally important to determine the correct sample type: host cell culture supernatant, cell pellets, or others.

Here, we tested HCP antibody coverage of the same antibody against CHO cell supernatant (CHO-S) and CHO cell pellets (CHO-P). A striking difference is observed: 97.1% for CHO-S and 70.0% for CHO-P.

If you are interested, feel free to contact our support team with a brief description of your project. Our scientists will reach out to you shortly.

Featured Publication: Tissue Barriers

July 20, 2022

Fig 1b: 2-D gel separation of proteins present in non-magnetic and magnetic capture membrane fractions with location of spot 16 identified by mass spectrometry as GRP75.

A recent publication in Tissue Barriers used our 2D DIGE / Mass Spec platform to identify key proteins in toxin-induced cell death.

Cholix is an exotoxin which induces cell death after crossing the epithelium. After comparing the protein profile of Cholix magnetic capture samples to the whole cell membrane (control) by 2D DIGE, 45 proteins showed significant differences. All 45 proteins were identified by mass spectrometry. One of the most over-expressed proteins in Cholix magnetic capture samples was GRP75 (spot 16).

GRP75, a heat shock protein that plays an important role in vesicles, was found to interact with Cholix during its crossing of the epithelium. Following endocytosis, GRP75 limits the delivery of Cholix to lysosomes.

More importantly, the binding of GRP75 to Cholix was further confirmed by 2 other platforms: IP and ELISA.

Comments on the data: With the fast development of proteomics technologies, researchers are often facing the challenge of identifying ‘real’ biomarkers that can be validated by other platforms. The findings in this paper proved again that 2D DIGE is a reliable platform to analyze complex proteomes and identify biomarkers.

If you are interested, feel free to contact our support team with a brief description of your project. Our scientists will reach out to you shortly.

Featured Service: Improved HCP antibody coverage protocol

June 15, 2022

Brown: 3015 spots detected by anti-CHO HCP antibody
Blue: 83 spots not detected by antibody
Anti-CHO HCP antibody coverage: 97.3%

One main challenge in performing HCP antibody coverage by 2D Western blot is the unspecific antibody binding, which causes high background and inaccuracy in quantitation. To address this issue, Applied Biomics has performed extensive internal studies testing different conditions.

With our recent improvements to the protocol, we were able to achieve:

  1. Significantly lower background
  2. Overall higher HCP antibody coverage

Featured Publication: bioRxiv

May 18, 2022

Study protein distribution within cells using our mass spectrometry service. A recent bioRxiv paper used Mass Spectrometry to identify proteins in various sections of a giant cell. Findings revealed that 30% of the Stentor proteome has polarized localization.

Featured Service: PTMs by Mass Spectrometry

April 19, 2022

Interested in PTM sites?

Our mass spectrometry service can identify PTM sites such as phosphorylation, acetylation, methylation (and more) with high sensitivity and accuracy. The service also includes the confirmation by MS/MS spectrum showing the presence of signature ions (if applicable).

Featured Publication: Int J Biol Sci

March 16, 2022

Identify important breast cancer biomarkers using our 2D DIGE service

An Int J Biol Sci study demonstrated the up-regulation of G6PD in doxorubicin-resistant breast cancer cells. Findings suggest that G6PD inhibition can be a promising strategy against this cancer.

Featured Publication: Cell

February 16, 2022

Quantitative neuronal proteome analysis using our iTRAQ services:

A study published in Cell quantifies proteome changes between control and chaperone-mediated autophagy (CMA) deficient mice using iTRAQ. The findings showed that functional CMA is essential for neuronal proteostasis to prevent neuro-degeneration.

New Year’s Promotion

January 11, 2022

New Year’s promotion: 10% Off Protein Identification (Offer Ends 02/28/2022)

Best wishes for a prosperous 2022! We would like to show our appreciation for choosing us to perform your proteomics service.

Take advantage of this limited-time discount to kick start your #research for the new year. The offer applies to all our loyal existing customers as well as new customers!

Terms: Only applies for Protein ID by standard MALDI-TOFTOF. Does not apply to previous orders. Applied Biomics’ service terms and conditions apply

Featured Publication: PLoS Pathogens

November 16, 2021

Determine important viral pathway proteins with our NanoLC-MS/MS services.

A recent PLoS study used our NanoLC-MS/MS service to identify critical binding proteins in the translation of the Andes orthohantavirus SmRNA.

Featured Service: PhosphoProteomics

October 18, 2021

Interested in Kinase/Phosphatase substrate mapping?

Our PhosphoProteomics platform can detect and quantify protein phosphorylation with high sensitivity and accuracy. We offer 3 study designs to fit your project needs.

News Release: Applied Biomics Offers Qualitative and Quantitative Host Cell Protein (HCP) Profiling Analysis

February 19, 2020

From Business Wire:

Applied Biomics, Inc., a leading Proteomics service provider, adds a HCP profiling analysis using their well established high resolution, large format 2-Dimensional Differential In-Gel Electrophoresis (2D DIGE) platform. This assay can be used to monitor HCP contamination during the purification process thus helps to improve purification strategy, specifically:

1) Visualize and overlay the HCP images between different samples
2) Quantitate HCP content by spot counting and PPM for each sample
3) Quantitate the purity of product or drug substance (DS) by spot counting and PPM
4) Quantitatively compare HCP content by fold-change and overall similarity between samples