Mass Spectrometry Service
Our Mass Spectrometry facility has over 15 years of experience handling all sample types for applications ranging from protein identification, quantitation, PTMs to biopharma related analysis.
We have optimized mass spectrometry sample preparation (cells, tissues, protein extract, serum, IP, etc), liquid chromatography and mass spectrometry scan settings for each application. By employing top-of-the-line Orbitrap Exploris technology, we offer low cost, high sensitivity and high accuracy Mass Spectrometry analysis.
Please use the table below to guide you in choosing the right service:
|Mass Spec Applications||Sample Types||Study Objectives||Data Report|
|Protein Identification 1|
|nanoLC-MS/MS||All types||Identify all proteins||x||Opt.||Opt.|
|MALDI-TOF/TOF||1D/2D bands||Identify top protein hit||x||Opt.|
|Protein Identification & Quantitation 2|
|Label Free Quan (LFQ)||All types||ID & quantify all proteins||x||x||Opt.|
|Data Independent Acquisition (DIA)||All types||ID & quantify all proteins||x||x|
|Single Cell Proteomics||Cells||ID & quantify proteins in a single cell||x||x||Opt.|
|iTRAQ Quantitation||All types||ID & quantify proteins by iTRAQ label||x||x||Opt.|
|TMT Quantitation||All types||ID & quantify proteins by TMT label||x||x||Opt.|
|Post Translational Modification Sites (PTMs) 3|
|Phospho-Sites||Purified / IP||Identify Phospho sites||x||x|
|Other PTM sites||Purified / IP||Identify PTM sites||x||x|
|BioPharma Applications 4|
|Intact Mass Analysis||Purified / Powder||Quantify intact protein masses||x||x|
|Peptide mapping||Purified||Confirm peptide sequence||x||Opt.|
|HCP Identification & Quan||HCP||ID & quantify HCPs and DS||x||x||Opt.|
1. Protein Identification
We offer two workflows for protein identification: nanoLC-MS/MS and MALDI-TOF/TOF
- In nanoLC-MS/MS, digested peptides from protein mixtures are first separated in a nano-flow LC, then subject to high-resolution orbitrap mass spectrometry. This approach is ideal for identifying all proteins in a protein mixture ranging from 1D gel bands to cell lysates.
- In MALDI-TOF/TOF, digested peptides from a single protein are directly subjected to peptide finger print (MS) followed by fragmentation (MSMS) of top precursors. This approach is ideal for identifying a single protein in 2D gel spots selected from upstream 2D DIGE analysis.
2. Protein Identification and Quantitation
We offer both label-free quantitation (LFQ) and labeled quantitation (iTRAQ, TMT). We have optimized both workflows along with our high-resolution mass spectrometry to ensure the highly sensitive detection and accurate quantitation of all proteins in each sample.
We offer both data-dependent analysis (DDA) by Proteome Discoverer and data-independent analysis (DIA) analysis by DIA-NN.
3. Post-translational Modification (PTM) site identifications
We offer PTM site identification services for the popular PTMs such as phosphorylation, acetylation, and methylation. Other types of PTMs are also covered.
4. BioPharma Applications
We have optimized the sample preparations and mass spectrometry settings for each work flow: Intact mass analysis, peptide mapping and HCP identification and quantitation.